Phanerochaete chrysosporium soluble proteome as a prelude for the analysis of heavy metal stress response.
Identifieur interne : 000722 ( Main/Exploration ); précédent : 000721; suivant : 000723Phanerochaete chrysosporium soluble proteome as a prelude for the analysis of heavy metal stress response.
Auteurs : Servet Ozcan [Turquie] ; Volkan Yildirim ; Levent Kaya ; Dirk Albrecht ; Dörte Becher ; Michael Hecker ; Gulay OzcengizSource :
- Proteomics [ 1615-9853 ] ; 2007.
Descripteurs français
- KwdFr :
- Cadmium (métabolisme), Cuivre (métabolisme), Données de séquences moléculaires (MeSH), Phanerochaete (composition chimique), Phanerochaete (génétique), Phanerochaete (métabolisme), Protéines fongiques (analyse), Protéines fongiques (classification), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Protéome (analyse), Signaux de triage des protéines (MeSH), Spectrométrie de masse MALDI (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
- analyse : Protéines fongiques, Protéome.
- classification : Protéines fongiques.
- composition chimique : Phanerochaete.
- génétique : Phanerochaete, Protéines fongiques.
- métabolisme : Cadmium, Cuivre, Phanerochaete, Protéines fongiques.
- Données de séquences moléculaires, Signaux de triage des protéines, Spectrométrie de masse MALDI, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Cadmium (metabolism), Copper (metabolism), Electrophoresis, Gel, Two-Dimensional (MeSH), Fungal Proteins (analysis), Fungal Proteins (classification), Fungal Proteins (genetics), Fungal Proteins (metabolism), Molecular Sequence Data (MeSH), Phanerochaete (chemistry), Phanerochaete (genetics), Phanerochaete (metabolism), Protein Sorting Signals (MeSH), Proteome (analysis), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH).
- MESH :
- chemical , analysis : Fungal Proteins, Proteome.
- chemical , classification : Fungal Proteins.
- chemical , genetics : Fungal Proteins.
- chemical , metabolism : Cadmium, Copper, Fungal Proteins.
- chemistry : Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- Electrophoresis, Gel, Two-Dimensional, Molecular Sequence Data, Protein Sorting Signals, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
A 2-D reference map in pI range 3-10 was constructed for the soluble protein fraction of Phanerochaete chrysosporium growing vegetatively under standard conditions. Functional annotation could be made for 517 spots out of 720 that were subjected to MALDI-TOF-MS analysis, according to the specific accession numbers from the P. chrysosporium genomic database. Further analysis of the data revealed 314 distinct ORFs, 118 of which yielded multiple spots on the master gel. Functional classification of the proteins was made according to the eukaryote orthologous groups defined in the organism's genome website. The functional class of PTMs, protein turnover and chaperones was represented with the highest number (63) of the identified ORFs. Six proteins were assigned to the hypothetical proteins and 29 were predicted to have a signal peptide sequence. Subcellular localization predictions were also made for the identified proteins. Of the protein spots detected on the master gel, 380 were found to be probably phosphorylated and 96 of these matched to the identified proteins. The reference map was efficiently used in the identification of the proteins differentially expressed under cadmium and copper stress. Three new ribosomal proteins as well as zinc-containing alcohol dehydrogenase, glucose-6-phosphate isomerase, flavonol/cinnamoyl-CoA reductase, H+-transporting two-sector ATPase, ribosomal protein S7, ribosomal protein S21e, elongation factor EF-1 alpha subunit were demonstrated as the most strongly induced.
DOI: 10.1002/pmic.200600526
PubMed: 17366474
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Ozcan, Servet" sort="Ozcan, Servet" uniqKey="Ozcan S" first="Servet" last="Ozcan">Servet Ozcan</name>
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<term>Fungal Proteins (analysis)</term>
<term>Fungal Proteins (classification)</term>
<term>Fungal Proteins (genetics)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phanerochaete (chemistry)</term>
<term>Phanerochaete (genetics)</term>
<term>Phanerochaete (metabolism)</term>
<term>Protein Sorting Signals (MeSH)</term>
<term>Proteome (analysis)</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Cadmium (métabolisme)</term>
<term>Cuivre (métabolisme)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Phanerochaete (composition chimique)</term>
<term>Phanerochaete (génétique)</term>
<term>Phanerochaete (métabolisme)</term>
<term>Protéines fongiques (analyse)</term>
<term>Protéines fongiques (classification)</term>
<term>Protéines fongiques (génétique)</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Protéome (analyse)</term>
<term>Signaux de triage des protéines (MeSH)</term>
<term>Spectrométrie de masse MALDI (MeSH)</term>
<term>Électrophorèse bidimensionnelle sur gel (MeSH)</term>
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<term>Proteome</term>
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<term>Copper</term>
<term>Fungal Proteins</term>
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<term>Protéome</term>
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<term>Molecular Sequence Data</term>
<term>Protein Sorting Signals</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
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<term>Signaux de triage des protéines</term>
<term>Spectrométrie de masse MALDI</term>
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<front><div type="abstract" xml:lang="en">A 2-D reference map in pI range 3-10 was constructed for the soluble protein fraction of Phanerochaete chrysosporium growing vegetatively under standard conditions. Functional annotation could be made for 517 spots out of 720 that were subjected to MALDI-TOF-MS analysis, according to the specific accession numbers from the P. chrysosporium genomic database. Further analysis of the data revealed 314 distinct ORFs, 118 of which yielded multiple spots on the master gel. Functional classification of the proteins was made according to the eukaryote orthologous groups defined in the organism's genome website. The functional class of PTMs, protein turnover and chaperones was represented with the highest number (63) of the identified ORFs. Six proteins were assigned to the hypothetical proteins and 29 were predicted to have a signal peptide sequence. Subcellular localization predictions were also made for the identified proteins. Of the protein spots detected on the master gel, 380 were found to be probably phosphorylated and 96 of these matched to the identified proteins. The reference map was efficiently used in the identification of the proteins differentially expressed under cadmium and copper stress. Three new ribosomal proteins as well as zinc-containing alcohol dehydrogenase, glucose-6-phosphate isomerase, flavonol/cinnamoyl-CoA reductase, H+-transporting two-sector ATPase, ribosomal protein S7, ribosomal protein S21e, elongation factor EF-1 alpha subunit were demonstrated as the most strongly induced.</div>
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<Abstract><AbstractText>A 2-D reference map in pI range 3-10 was constructed for the soluble protein fraction of Phanerochaete chrysosporium growing vegetatively under standard conditions. Functional annotation could be made for 517 spots out of 720 that were subjected to MALDI-TOF-MS analysis, according to the specific accession numbers from the P. chrysosporium genomic database. Further analysis of the data revealed 314 distinct ORFs, 118 of which yielded multiple spots on the master gel. Functional classification of the proteins was made according to the eukaryote orthologous groups defined in the organism's genome website. The functional class of PTMs, protein turnover and chaperones was represented with the highest number (63) of the identified ORFs. Six proteins were assigned to the hypothetical proteins and 29 were predicted to have a signal peptide sequence. Subcellular localization predictions were also made for the identified proteins. Of the protein spots detected on the master gel, 380 were found to be probably phosphorylated and 96 of these matched to the identified proteins. The reference map was efficiently used in the identification of the proteins differentially expressed under cadmium and copper stress. Three new ribosomal proteins as well as zinc-containing alcohol dehydrogenase, glucose-6-phosphate isomerase, flavonol/cinnamoyl-CoA reductase, H+-transporting two-sector ATPase, ribosomal protein S7, ribosomal protein S21e, elongation factor EF-1 alpha subunit were demonstrated as the most strongly induced.</AbstractText>
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<name sortKey="Kaya, Levent" sort="Kaya, Levent" uniqKey="Kaya L" first="Levent" last="Kaya">Levent Kaya</name>
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